{"product_id":"2x-m4-taq-master-mix-with-dye","title":"HoppeSyler 2X M4 Taq Master Mix with Dye","description":"\u003cp\u003e\u003cstrong\u003eHoppeSyler 2X M4 \u003ci\u003eTaq\u003c\/i\u003e Master Mix with Dye\u003c\/strong\u003e is a ready-to-use 2X master mix formulated for fast PCR, high yield and strong specificity in standard endpoint workflows. It contains M4 \u003ci\u003eTaq\u003c\/i\u003e DNA Polymerase, dNTPs, MgCl₂, reaction buffer, stabilizers and a blue tracking dye in a single tube, reducing hands-on time and pipetting error. M4 is supplied only in hot start format: polymerase activity is suppressed during reaction setup and released under cycling (hot start is inherent to the engineered enzyme; no separate activation step), which reduces non-specific amplification and primer-dimer formation. Suited to rapid, reliable PCR from bacterial colonies, overnight cultures, crude-lysate genotyping samples, soil-associated samples and other routine templates; reactions load directly onto the gel.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003e🍁 Researched, Developed and Manufactured in London, ON, Canada.\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eIncluded in the Kit\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e2X M4 \u003ci\u003eTaq\u003c\/i\u003e Master Mix with Dye (4 × 1.25 mL)\u003c\/li\u003e\n\u003cli\u003eGC Enhancer (1 × 1 mL)\u003c\/li\u003e\n\u003cli\u003eDatasheet with protocol (PDF)\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eGC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C.\u003c\/em\u003e\u003c\/p\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCatalogue Number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eNumber of Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eM0802-020\u003c\/td\u003e\n\u003ctd\u003e200\u003c\/td\u003e\n\u003ctd\u003e5.0 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHot start in a ready-to-use 2X format\u003c\/li\u003e\n\u003cli\u003eFast cycling: 15 s per kb extension\u003c\/li\u003e\n\u003cli\u003eTolerates common PCR inhibitors, including humic acid\u003c\/li\u003e\n\u003cli\u003eDirect PCR from bacterial colonies and cultures\u003c\/li\u003e\n\u003cli\u003eBlue tracking dye for direct gel loading\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cb\u003eLot-release testing:\u003c\/b\u003e the polymerase is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity before formulation; each master mix lot is tested before release.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hoppesyler.com\/datasheets\/HSS-MM03-DS-v1.0.pdf\" target=\"_blank\" rel=\"noopener\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hoppesyler.com\/sds\/HSS-MM03-SDS-v1.0.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSafety Data Sheet, 2X M4 Taq Master Mix with Dye (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.hoppesyler.com\/sds\/HSS-PL-GCE-SDS-v1.0.pdf\" target=\"_blank\" rel=\"noopener\"\u003eSafety Data Sheet, GC Enhancer (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e","brand":"HoppeSyler","offers":[{"title":"200 rxn","offer_id":54935090430274,"sku":"CSR00018|M0802-020","price":119.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/mm03-takeA_pro_2K_2-3.png?v=1789686133","url":"https:\/\/www.hoppesylerreagents.com\/products\/2x-m4-taq-master-mix-with-dye","provider":"HoppeSyler Reagents","version":"1.0","type":"link"}