{"product_id":"hot-start-taq-dna-polymerase-with-10x-reaction-buffer-no-dntps","title":"HoppeSyler Hot Start Taq DNA Polymerase","description":"\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eHoppeSyler Hot Start Taq DNA Polymerase \u003c\/strong\u003ereliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cem\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/em\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cem\u003e\u003cstrong\u003eHot Start Technology\u003c\/strong\u003e\u003c\/em\u003e\u003c\/div\u003e\n\u003cdiv\u003eHot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cstrong\u003e🍁 Researched, Developed and Manufactured in London, ON, Canada.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cbr\u003e\n\u003ctable width=\"100%\" style=\"height: 107px; width: 74.6429%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 38.6946%;\"\u003e\u003cstrong\u003e Catalogue Number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 18.1818%;\"\u003e\u003cstrong\u003eUnits\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 42.1911%;\"\u003e\u003cstrong\u003e\u003cmeta charset=\"utf-8\"\u003eNumber of Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 38.6946%;\"\u003eM0202-010\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 18.1818%;\"\u003e1000 U\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 42.1911%;\"\u003e400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 38.6946%;\"\u003eM0202-050\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 18.1818%;\"\u003e5000 U\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 42.1911%;\"\u003e2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 38.6946%;\"\u003eM0202-500\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 18.1818%;\"\u003e50,000 U\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; width: 42.1911%;\"\u003e20,000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003eIncluded in the Kit\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cul\u003e\n\u003cli\u003e10X PCR Buffer\u003c\/li\u003e\n\u003cli\u003e50 mM Magnesium Sulfate\u003c\/li\u003e\n\u003cli\u003eGC Extender (For GC-rich templates and difficult templates)\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eProtocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003e\u003cspan lang=\"EN-US\"\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cul\u003e\n\u003cli\u003eGenerates PCR product with 3'-dA overhangs\u003c\/li\u003e\n\u003cli\u003eThe error rate in PCR is \u003cspan lang=\"EN-US\"\u003e2.2 x 10\u003csup\u003e-5 \u003c\/sup\u003e\u003c\/span\u003eerrors per nucleotide per cycle\u003c\/li\u003e\n\u003cli\u003eGenerates PCR product of up to 5 kb\u003c\/li\u003e\n\u003cli\u003eAptamer based Hot Start provides a reversible inhibition of non-specific PCR products\u003c\/li\u003e\n\u003cli\u003eStable at room temperature for up to 2 weeks\u003cmeta charset=\"utf-8\"\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cmeta charset=\"utf-8\"\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cul\u003e\n\u003cli\u003eEffective in routine and high throughput PCR\u003c\/li\u003e\n\u003cli\u003eIncorporates modified nucleotides in PCR\u003c\/li\u003e\n\u003cli\u003eEffective in DNA labeling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\n\n\u003cbr\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"HoppeSyler","offers":[{"title":"400 rxn","offer_id":50522645102914,"sku":"CSR00002|M0202-010","price":99.0,"currency_code":"CAD","in_stock":true},{"title":"2000 rxn","offer_id":50522645135682,"sku":"CSR00002|M0202-050","price":249.0,"currency_code":"CAD","in_stock":true},{"title":"20000 rxn","offer_id":50522645168450,"sku":"CSR00002|M0202-500","price":699.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/hoppesyler-hot-start-taq-dna-polymerase.png?v=1779471193","url":"https:\/\/www.hoppesylerreagents.com\/products\/hot-start-taq-dna-polymerase-with-10x-reaction-buffer-no-dntps","provider":"HoppeSyler Reagents","version":"1.0","type":"link"}