{"product_id":"midori-green-advanced-1ml","title":"Midori Green Advanced Stain, 1 mL","description":"\u003cp style=\"margin: 0cm;\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/BC_Upload_5dcb7023-cc5d-49f9-942c-f1acb4d496cc.jpg?v=1769027341\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eA safer alternative to ethidium bromide\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eMIDORI Green Advance stains single- and double-stranded nucleic acids with sensitivity comparable to ethidium bromide (EtBr). Like EtBr, it’s added directly to molten agarose during gel casting, making it an easy drop-in swap for most existing workflows.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eYou can still use standard UV transilluminators and common filter sets, but MIDORI Green Advance performs best with blue LED and especially Blue\/Green LED illuminators and gel documentation systems. These visible-light systems are a great pairing because you can work without face shields or goggles when imaging or excising bands. Just as importantly, blue\/green light does not cross-link DNA, helping preserve DNA integrity for downstream steps like cloning.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eMIDORI Green Advance is highly sensitive, including for small DNA fragments, and can be used at dilutions up to 1:25,000. Practically, 4–6 µL is sufficient for a 100 mL agarose gel, so a 1 mL tube can stain roughly 17–25 litres of agarose gel.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/Midori-Green-Xtra-Cost-Adv.png?v=1772146768\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eThoroughly evaluated for lab safety\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eMIDORI Green Advance is positioned as a non-toxic, non-mutagenic alternative to traditional EtBr. It has been assessed using multiple safety assays. Compared with EtBr (a known strong mutagen), MIDORI Green Advance shows substantially lower mutagenicity in the Ames test, a standard screen for mutagenic potential. It also reports negative results in additional genotoxicity testing (including assays such as the mouse bone marrow micronucleus test and chromosome aberration test), supporting its classification as non-carcinogenic in these test contexts.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eOn the toxicity side, MIDORI Green stains are described as non-toxic and are often handled as non-hazardous waste under typical lab disposal procedures (unlike EtBr and some other “safe” dyes that still require hazardous handling). For users who want extra reassurance, MIDORI Green Advance is also reported to be resistant to latex\/nitrile glove penetration even after extended exposure (e.g., 6 hours).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eThree MIDORI Green options, tuned to different workflows\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eThe MIDORI Green family includes three related green-fluorescent stains designed for different use cases:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-indent: -36.0pt; mso-list: l0 level2 lfo1; margin: 0cm 0cm 0cm 90.0pt;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: Arial; color: black; mso-themecolor: text1;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e•\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan dir=\"LTR\"\u003e\u003c\/span\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eMIDORI Green Advance: Added to molten agarose before casting (EtBr-style). Delivers strong signal-to-noise and reliable sensitivity, especially when visualized with blue or Blue\/Green LEDs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-indent: -36.0pt; mso-list: l0 level2 lfo1; margin: 0cm 0cm 0cm 90.0pt;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: Arial; color: black; mso-themecolor: text1;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e•\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan dir=\"LTR\"\u003e\u003c\/span\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eMIDORI Green Direct: Mixed directly into samples and loaded onto an unstained gel. Includes loading dye and provides sensitivity similar to (or slightly better than) Advance.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-indent: -36.0pt; mso-list: l0 level2 lfo1; margin: 0cm 0cm 0cm 90.0pt;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: Arial; color: black; mso-themecolor: text1;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e•\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e                \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan dir=\"LTR\"\u003e\u003c\/span\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eMIDORI Green Xtra\/Easy: Added to molten agarose (and buffer). Optimized for Blue\/Green and blue LED excitation, delivering very high fluorescence while minimizing background because it does not stain the agarose itself. This yields excellent signal-to-noise and very sensitive detection of DNA\/RNA, often described as an upgrade over EtBr.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eAdvance and Direct can be used with UV, though they are typically less efficient under UV than under visible LED excitation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eSafer imaging, better cloning outcomes\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eUsing MIDORI Green dyes with blue\/green LED illumination can improve downstream cloning success because it avoids UV-induced DNA damage. UV exposure during band excision can rapidly create lesions (e.g., thymine dimers) that reduce ligation and transformation efficiency, with noticeable declines after seconds to tens of seconds of exposure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e \u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/EtBr_MidoriGreenDirect_transformed-3.jpg?v=1772146009\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm; text-align: center;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003e\u003cstrong\u003eMIDORI green can boost your cloning results\u003c\/strong\u003e!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm; text-align: center;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; mso-fareast-font-family: 'DengXian Light'; mso-fareast-theme-font: major-fareast; color: black; mso-themecolor: text1;\"\u003eIn a representative cloning experiment (restriction digest, gel separation, band excision, silica cleanup, ligation, and transformation), gels stained with MIDORI Green Direct and visualized under Blue\/Green LEDs yielded markedly more positive transformants compared with EtBr stained gels viewed under UV. Even if a lab continues to use EtBr, switching from UV to Blue\/Green LED illumination can immediately help preserve DNA integrity and improve cloning efficiency.\u003c\/span\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: black; mso-themecolor: text1;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: black; mso-themecolor: text1;\"\u003eDocuments\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/MG10_Midori-Green-Xtra_Manual.pdf?v=1772146292\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: black; mso-themecolor: text1;\"\u003eProtocol\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: black; mso-themecolor: text1;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/MSDS-Midori-Green-Xtra-MG10.pdf?v=1772146292\"\u003eMSDS\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/Midori-Green-Xtra-Safety-Report.pdf?v=1772146292\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif; color: black; mso-themecolor: text1;\"\u003eSafety Report\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"margin: 0cm;\"\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e","brand":"BullDog Bio","offers":[{"title":"Default Title","offer_id":51865742639426,"sku":"HS-MG04","price":189.63,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/TUBE_IMAGE.png?v=1772146768","url":"https:\/\/www.hoppesylerreagents.com\/products\/midori-green-advanced-1ml","provider":"HoppeSyler Reagents","version":"1.0","type":"link"}