{"product_id":"robust-dna-polymerase-with-10x-robust-pcr-buffer-no-dntps","title":"HoppeSyler ROBUST DNA Polymerase","description":"\u003cp\u003e\u003cstrong\u003eHoppeSyler ROBUST DNA Polymerase\u003c\/strong\u003e is a highly efficient next-generation DNA polymerase offering enhanced processivity and yield compared to Taq DNA polymerase. This enzyme enables shorter extension times and fewer cycles to achieve equivalent levels of amplification. It also features significantly improved resistance to various PCR inhibitors, chemical denaturants, and increased thermal stability, maintaining activity even after 1 hour at 95 °C. Its enhanced robustness allows for the use of lower-quality template DNA and simplifies DNA purification protocols. The ROBUST DNA Polymerase utilizes aptamer-based hot start technology, providing reversible inhibition that prevents primer-dimer formation and non-specific amplification, enabling reaction setup at room temperature.\u003c\/p\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\u003cem\u003e\u003cstrong\u003eHot-Start Technology\u003c\/strong\u003e\u003c\/em\u003e\u003c\/div\u003e\n\u003cdiv\u003eThe HoppeSyler ROBUST DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3' cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e🍁\u003cstrong\u003e Researched, Developed and Manufactured in London, ON, Canada.\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\u003cstrong\u003eIncluded in the Kit\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cul\u003e\n\u003cli\u003eHoppeSyler ROBUST DNA Polymerase\u003c\/li\u003e\n\u003cli\u003e10X ROBUST PCR Buffer\u003c\/li\u003e\n\u003cli\u003e50 mM Magnesium Sulfate\u003c\/li\u003e\n\u003cli\u003eGC Extender (For GC-rich or difficult to amplify templates)\u003c\/li\u003e\n\u003cli\u003eProtocol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ctable style=\"width: 70.7143%;\" height=\"106\" width=\"396\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0722%;\"\u003e\u003cstrong\u003e Catalogue Number\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 15.343%;\"\u003e\u003cstrong\u003eUnits\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 43.6823%;\"\u003e\u003cstrong\u003e\u003cmeta charset=\"utf-8\"\u003eNumber of Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0722%;\"\u003eM0301-002\u003c\/td\u003e\n\u003ctd style=\"width: 15.343%;\"\u003e200 U\u003c\/td\u003e\n\u003ctd style=\"width: 43.6823%;\"\u003e80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0722%;\"\u003eM0301-010\u003c\/td\u003e\n\u003ctd style=\"width: 15.343%;\"\u003e1000 U\u003c\/td\u003e\n\u003ctd style=\"width: 43.6823%;\"\u003e400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0722%;\"\u003eM0301-050\u003c\/td\u003e\n\u003ctd style=\"width: 15.343%;\"\u003e5000 U\u003c\/td\u003e\n\u003ctd style=\"width: 43.6823%;\"\u003e2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cspan lang=\"EN-US\"\u003e\u003cspan lang=\"EN-US\"\u003e\u003cmeta charset=\"utf-8\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003e\u003cspan lang=\"EN-US\"\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cul\u003e\n\u003cli\u003eRobust and reliable reactions\u003c\/li\u003e\n\u003cli\u003eCompatible with contaminated templates, such as plant DNA\u003c\/li\u003e\n\u003cli\u003eCompatible with direct colony PCR\u003c\/li\u003e\n\u003cli\u003e2-3 X faster speeds in PCR reactions (15 - 30 sec\/ kb)\u003c\/li\u003e\n\u003cli\u003eTolerates a wide range of templates\u003c\/li\u003e\n\u003cli\u003eAmplification confirmed up to 5 kb in PCR reactions\u003c\/li\u003e\n\u003cli\u003eThe error rate in PCR is 2.2 x 10\u003cmeta charset=\"utf-8\"\u003e\n\u003cspan lang=\"EN-US\"\u003e\u003csup\u003e-5 \u003c\/sup\u003e\u003c\/span\u003eerrors per nucleotide per cycle\u003c\/li\u003e\n\u003cli\u003eHot Start technology prevents the formation of non-specific products in PCR\u003c\/li\u003e\n\u003cli\u003eStable at room temperature for up to 2 weeks\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\n\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"HoppeSyler","offers":[{"title":"80 rxn","offer_id":50522645332290,"sku":"CSR00005|M0301-002","price":45.0,"currency_code":"CAD","in_stock":true},{"title":"400 rxn","offer_id":50522645365058,"sku":"CSR00005|M0301-010","price":79.0,"currency_code":"CAD","in_stock":true},{"title":"2000 rxn","offer_id":50522645397826,"sku":"CSR00005|M0301-050","price":275.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0668\/7719\/4562\/files\/hoppesyler-robust-dna-polymerase.png?v=1779471183","url":"https:\/\/www.hoppesylerreagents.com\/products\/robust-dna-polymerase-with-10x-robust-pcr-buffer-no-dntps","provider":"HoppeSyler Reagents","version":"1.0","type":"link"}