HoppeSyler 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The HoppeSyler Hot Start Taq Master Mix offers exceptional value per unit in terms of cost. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.   🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Number of Reactions Volume (mL) M0602-005 50 1.25 M0602-020 200 5.0   Included in the Kit HoppeSyler 2X Hot Start Taq Master Mix GC Enhancer (For GC-rich or difficult to amplify templates) Protocol Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle Hot-Start technology prevents the formation of non-specific products in PCR For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler dNTP Mix (10 mM each) is a ready-to-use aqueous solution of dATP, dTTP, dCTP and dGTP at a concentration of 10 mM. The individual bases are at a stock concentration of 100 mM, and then are mixed in RNase/DNase/Protease free water to achieve indicated concentrations. The purity of these bases is > 99 %, as verified by HPLC.   🍁 Researched, Developed and Manufactured in London, ON, Canada. Storage conditions  All components are to be stored at -20 °C. These components can be thawed at 4 °C prior to use.   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA, producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis. 🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Units Number of Reactions M0202-010 1000 U 400 M0202-050 5000 U 2000 M0202-500 50,000 U 20,000   Included in the Kit 10X PCR Buffer 50 mM Magnesium Sulfate GC Enhancer (For GC-rich or difficult to amplify templates) Protocol Features Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products Stable at room temperature for up to 2 weeks Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR Effective in DNA labeling For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler 2X Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The HoppeSyler Taq Master Mix offers exceptional value per unit in terms of cost.  🍁 Researched, Developed and Manufactured in London, ON, Canada.   Included in the Kit HoppeSyler 2X Taq Master Mix GC Enhancer (For GC-rich or difficult to amplify templates) Protocol  Catalogue Number Number of Reactions Volume (mL) M0501-005 50 1.25 M0501-020 200 5.0 Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler 2X M4 Taq Master Mix with Dye is a ready-to-use 2X master mix formulated for fast PCR, high yield and strong specificity in standard endpoint workflows. It contains M4 Taq DNA Polymerase, dNTPs, MgCl₂, reaction buffer, stabilizers and a blue tracking dye in a single tube, reducing hands-on time and pipetting error. M4 is supplied only in hot start format: polymerase activity is suppressed during reaction setup and released under cycling (hot start is inherent to the engineered enzyme; no separate activation step), which reduces non-specific amplification and primer-dimer formation. Suited to rapid, reliable PCR from bacterial colonies, overnight cultures, crude-lysate genotyping samples, soil-associated samples and other routine templates; reactions load directly onto the gel.🍁 Researched, Developed and Manufactured in London, ON, Canada.Included in the Kit 2X M4 Taq Master Mix with Dye (4 × 1.25 mL) GC Enhancer (1 × 1 mL) Datasheet with protocol (PDF) GC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C. Catalogue Number Number of Reactions Volume M0802-020 200 5.0 mL Features Hot start in a ready-to-use 2X format Fast cycling: 15 s per kb extension Tolerates common PCR inhibitors, including humic acid Direct PCR from bacterial colonies and cultures Blue tracking dye for direct gel loading Lot-release testing: the polymerase is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity before formulation; each master mix lot is tested before release.Documents Datasheet (PDF) Safety Data Sheet, 2X M4 Taq Master Mix with Dye (PDF) Safety Data Sheet, GC Enhancer (PDF) For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler M4 Taq DNA Polymerase is an engineered, full-length thermostable DNA polymerase developed for fast, robust endpoint PCR across a wide range of sample types. It is supplied only in hot start format: the enzyme is inactive during reaction setup and becomes active under PCR cycling conditions, which improves specificity and reduces primer-dimer and non-specific amplification. M4 is optimized for fast cycling, high product yield and tolerance of common PCR inhibitors, and amplifies directly from bacterial colonies, overnight cultures, crude lysates and other challenging inputs.🍁 Researched, Developed and Manufactured in London, ON, Canada. Catalogue Number Units Number of Reactions M0902-010 1,000 U 400 M0902-050 5,000 U 2,000 Features Hot start built into the engineered enzyme: inactive during setup, fully active under cycling, no separate activation step Fast cycling: 15 s per kb extension; 2 kb amplified with a 20 s extension step Tolerates PCR inhibitors, including humic acid in soil extracts Direct PCR from bacterial colonies and overnight cultures Room-temperature stable up to 2 weeks Applications Colony PCR and direct PCR from culture Genotyping from crude lysates (for example mouse tail) Routine endpoint PCR Inhibitor-rich templates (soil-associated samples) Included in the Kit Component 1,000 U 5,000 U M4 Taq DNA Polymerase (5 U/µL) 1 × 200 µL 1 × 1 mL 5X M4 PCR Buffer (with MgCl₂) 4 × 1 mL 1 × 50 mL GC Enhancer 1 × 1 mL 1 × 10 mL Datasheet with protocol (PDF)GC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C.Lot-release testing: each polymerase lot is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity; lot-to-lot activity within ±5%.Documents Datasheet (PDF) Safety Data Sheet, M4 Taq DNA Polymerase (PDF) Safety Data Sheet, 5X M4 PCR Buffer (PDF) Safety Data Sheet, GC Enhancer (PDF) For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler ROBUST DNA Polymerase is a highly efficient next-generation DNA polymerase offering enhanced processivity and yield compared to Taq DNA polymerase. This enzyme enables shorter extension times and fewer cycles to achieve equivalent levels of amplification. It also features significantly improved resistance to various PCR inhibitors, chemical denaturants, and increased thermal stability, maintaining activity even after 1 hour at 95 °C. Its enhanced robustness allows for the use of lower-quality template DNA and simplifies DNA purification protocols. The ROBUST DNA Polymerase utilizes aptamer-based hot start technology, providing reversible inhibition that prevents primer-dimer formation and non-specific amplification, enabling reaction setup at room temperature. Hot-Start Technology The HoppeSyler ROBUST DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3' cap that prevents its amplification and ensures no interference with cloning or any downstream analysis. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Included in the Kit HoppeSyler ROBUST DNA Polymerase 10X ROBUST PCR Buffer 50 mM Magnesium Sulfate GC Enhancer (For GC-rich or difficult to amplify templates) Protocol  Catalogue Number Units Number of Reactions M0301-002 200 U 80 M0301-010 1000 U 400 M0301-050 5000 U 2000   Features Robust and reliable reactions Compatible with contaminated templates, such as plant DNA Compatible with direct colony PCR 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle Hot Start technology prevents the formation of non-specific products in PCR Stable at room temperature for up to 2 weeks For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Taq DNA Polymerase reliably amplifies a wide variety of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA, producing consistent PCR products. This Taq polymerase leaves a 3' adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. 🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Units Number of Reactions M0101-010 1000 U 400 M0101-050 5000 U 2000 M0101-500 50,000 U 20,000   Features Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Stable at room temperature for up to 2 weeks Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR Effective in DNA labeling Included in the Kit 10X PCR Buffer 50 mM Magnesium Sulfate GC Enhancer (For GC-rich or difficult to amplify templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler High Fidelity DNA Polymerase is a recombinant polymerase that contains a 5'->3' DNA polymerase and a 3'->5' exonuclease domains along with a Sso7D tethering domain. The High-Fidelity DNA polymerase achieves 55-fold higher fidelity than Taq DNA polymerase and generates blunt end PCR products. The High-Fidelity DNA polymerase can generate DNA fragments up to 10 Kbp in length with higher accuracy and two-to-three-fold greater processivity than Taq DNA polymerase. Source High Fidelity DNA Polymerase is a recombinant polymerase derived from the archaeon Pyrococcus furiosus. 🍁 Researched, Developed and Manufactured in London, ON, Canada.    Catalogue Number Units Number of Reactions M0401-002 200 U 80 M0401-010 1000 U 400 M0401-050 5000 U 2000   Included in the Kit HoppeSyler High Fidelity DNA Polymerase GC Enhancer (For GC-rich or difficult to amplify templates) 5X High Fidelity PCR Buffer Protocol Features High fidelity amplification (> 55X Higher than Taq) Robust and reliable amplification Tolerates a wide range of templates 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Resistant to contaminated templates, such as plant DNA Amplification confirmed up to 10 kb in PCR reactions Superior performance for a broad range of templates (High AT to high GC rich) For Research Use Only. Not for use in diagnostic procedures.

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