Description

HoppeSyler 2X M4 Taq Master Mix with Dye is a ready-to-use 2X master mix formulated for fast PCR, high yield and strong specificity in standard endpoint workflows. It contains M4 Taq DNA Polymerase, dNTPs, MgCl₂, reaction buffer, stabilizers and a blue tracking dye in a single tube, reducing hands-on time and pipetting error. M4 is supplied only in hot start format: polymerase activity is suppressed during reaction setup and released under cycling (hot start is inherent to the engineered enzyme; no separate activation step), which reduces non-specific amplification and primer-dimer formation. Suited to rapid, reliable PCR from bacterial colonies, overnight cultures, crude-lysate genotyping samples, soil-associated samples and other routine templates; reactions load directly onto the gel.

🍁 Researched, Developed and Manufactured in London, ON, Canada.

Included in the Kit

• 2X M4 Taq Master Mix with Dye (4 × 1.25 mL)
• GC Enhancer (1 × 1 mL)
• Datasheet with protocol (PDF)

GC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C.

Features

• Hot start in a ready-to-use 2X format
• Fast cycling: 15 s per kb extension
• Tolerates common PCR inhibitors, including humic acid
• Direct PCR from bacterial colonies and cultures
• Blue tracking dye for direct gel loading

Lot-release testing: the polymerase is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity before formulation; each master mix lot is tested before release.

Documents

• Datasheet (PDF)
• Safety Data Sheet, 2X M4 Taq Master Mix with Dye (PDF)
• Safety Data Sheet, GC Enhancer (PDF)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Species of Origin: Thermus aquaticus (engineered, recombinant)
• Enzyme Type: Engineered hot start thermostable DNA polymerase
• Hot Start: Inherent to the enzyme; no separate activation step
• Format: 2X Master Mix, includes dNTPs
• MgCl2: MgCl2
• Optimal Reaction Volume: 50 µL
• MgCl2 at 1X: 2.5 mM
• dNTPs at 1X: 0.2 mM each
• Extension Rate: 15 s / kb
• Loading Dye: Included, blue (bromophenol blue); runs at 300 to 500 bp depending on gel percentage
• GC Enhancer: Yes, included in a separate tube
• Inhibitor Tolerance: Tolerates common PCR inhibitors, including humic acid
• Lot-Release Testing: DNase activity, mycoplasma DNA, host genomic DNA
• PCR activity on the polymerase; each master mix lot tested before release: PCR activity on the polymerase; each master mix lot tested before release
• Storage: 1 year at -20 °C
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