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HoppeSyler High Fidelity DNA Polymerase

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    HoppeSyler High Fidelity DNA Polymerase is a recombinant polymerase that contains a 5'->3' DNA polymerase and a 3'->5' exonuclease domains... Read more

    $99.00

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      Description

      HoppeSyler High Fidelity DNA Polymerase is a recombinant polymerase that contains a 5'->3' DNA polymerase and a 3'->5' exonuclease domains along with a Sso7D tethering domain. The High-Fidelity DNA polymerase achieves 55-fold higher fidelity than Taq DNA polymerase and generates blunt end PCR products. The High-Fidelity DNA polymerase can generate DNA fragments up to 10 Kbp in length with higher accuracy and two-to-three-fold greater processivity than Taq DNA polymerase.

      Source
      High Fidelity DNA Polymerase is a recombinant polymerase derived from the archaeon Pyrococcus furiosus.

      🍁 Researched, Developed and Manufactured in London, ON, Canada.

       

       Catalogue Number Units Number of Reactions
      M0401-002 200 U 80
      M0401-010 1000 U 400
      M0401-050 5000 U 2000

       

      Included in the Kit
      • HoppeSyler High Fidelity DNA Polymerase
      • GC Extender (For GC-rich or difficult to amplify templates)
      • 5X High Fidelity PCR Buffer
      • Protocol
      Features
      • High fidelity amplification (> 55X Higher than Taq)
      • Robust and reliable amplification
      • Tolerates a wide range of templates
      • 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb)
      • Resistant to contaminated templates, such as plant DNA
      • Amplification confirmed up to 10 kb in PCR reactions
      • Superior performance for a broad range of templates (High AT to high GC rich)

      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      • Species of Origin: Pyrococcus furiosus
      • Enzyme Type: Thermostable DNA polymerase (Type B)
      • 5'->3' Exonuclease Activity: Yes
      • “A” Overhang: No. Blunt-end
      • 3'->5' Exonuclease Activity: Yes
      • Processivity Rate: 3 Kbp / minute
      • Amplification Limit: confirmed to 10 Kbp
      • Enzyme Concentration: 5 units/µL
      • Unit Definition: One unit incorporates 10 nmol of dNTP into acid-precipitable material in 30 minutes at 74°C.
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