Name: HoppeSyler Hot Start Taq DNA Polymerase
Brand: HoppeSyler
SKU: CSR00002|M0202-010
Price: 99.0 CAD
Rating: 5.0 (1 reviews)

HoppeSyler Hot Start Taq DNA Polymerase

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HoppeSyler Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing... Read more

Variant

400 rxn
2000 rxn
20000 rxn

$99.00

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Description

HoppeSyler Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.

Hot Start Technology

Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.

🍁 Researched, Developed and Manufactured in London, ON, Canada.

Catalogue Number	Units	Number of Reactions
M0202-010	1000 U	400
M0202-050	5000 U	2000
M0202-500	50,000 U	20,000

Included in the Kit

10X PCR Buffer
50 mM Magnesium Sulfate
GC Extender (For GC-rich templates and difficult templates)
Protocol

Features

Generates PCR product with 3'-dA overhangs
The error rate in PCR is 2.2 x 10^-5errors per nucleotide per cycle
Generates PCR product of up to 5 kb
Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products
Stable at room temperature for up to 2 weeks

Applications

Effective in routine and high throughput PCR
Incorporates modified nucleotides in PCR
Effective in DNA labeling

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species of Origin: Thermus aquaticus
Enzyme Type: Thermostable DNA polymerase
5’->3′ Exonuclease Activity: Yes
“A” Overhang: Yes
3’->5′ Exonuclease Activity: No
Processivity Rate: 1 Kbp / minute
Unit Definition: One unit incorporates 10 nmol of dNTP into acid-precipitable material in 30 minutes at 74°C.
Amplification Limit: Confirmed to 5 Kbp
Enzyme Concentration: 5 units/µL
Reaction Buffer (included): Yes. 10x concentration (no dNTPs). Magnesium sulfate is provided separately
GC Enhancer: Included
DNase Free: Yes
RNase Free: Yes
Protease Free: Yes
Mycoplasma gDNA Free: Yes, measured at <10 copies per unit of enzyme
E. coli gDNA Reduced: Yes, Measured at >10 copies per unit of enzyme
SYBR™ Green Compatible: Yes
Storage: 1 year at -20 °C
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Description

Specifications

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