Description

HoppeSyler M4 Taq DNA Polymerase is an engineered, full-length thermostable DNA polymerase developed for fast, robust endpoint PCR across a wide range of sample types. It is supplied only in hot start format: the enzyme is inactive during reaction setup and becomes active under PCR cycling conditions, which improves specificity and reduces primer-dimer and non-specific amplification. M4 is optimized for fast cycling, high product yield and tolerance of common PCR inhibitors, and amplifies directly from bacterial colonies, overnight cultures, crude lysates and other challenging inputs.

🍁 Researched, Developed and Manufactured in London, ON, Canada.

Features

• Hot start built into the engineered enzyme: inactive during setup, fully active under cycling, no separate activation step
• Fast cycling: 15 s per kb extension; 2 kb amplified with a 20 s extension step
• Tolerates PCR inhibitors, including humic acid in soil extracts
• Direct PCR from bacterial colonies and overnight cultures
• Room-temperature stable up to 2 weeks

Applications

• Colony PCR and direct PCR from culture
• Genotyping from crude lysates (for example mouse tail)
• Routine endpoint PCR
• Inhibitor-rich templates (soil-associated samples)

Included in the Kit

• Datasheet with protocol (PDF)

GC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C.

Lot-release testing: each polymerase lot is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity; lot-to-lot activity within ±5%.

Documents

• Datasheet (PDF)
• Safety Data Sheet, M4 Taq DNA Polymerase (PDF)
• Safety Data Sheet, 5X M4 PCR Buffer (PDF)
• Safety Data Sheet, GC Enhancer (PDF)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Species of Origin: Thermus aquaticus (engineered, recombinant)
• Enzyme Type: Engineered hot start thermostable DNA polymerase
• Format: Hot start only; inherent to the enzyme, no separate activation step
• Enzyme Concentration: 5 units / µL
• Unit Definition: One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74 °C
• Reaction Buffer (included): Yes. 5X M4 PCR Buffer, pH 8.8, contains 12.5 mM MgCl2 (2.5 mM at 1X); no dNTPs
• Storage Buffer: 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Brij-58, 50% glycerol
• Extension Rate: 15 s / kb
• Demonstrated Amplicon: 2.0 kb (lambda DNA, 20 s extension step)
• Inhibitor Tolerance: Tolerates PCR inhibitors, including humic acid in soil extracts
• GC Enhancer: Yes, included
• Room-Temperature Stability: Up to 2 weeks
• Lot-Release Testing: DNase activity, mycoplasma DNA, host genomic DNA
• PCR activity; lot-to-lot activity within ±5%: PCR activity; lot-to-lot activity within ±5%
• Storage: 1 year at -20 °C
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