The HoppeSyler Mammalian RNA Purification Kit provides a rapid, robust method to purify high-quality total RNA from mammalian cell cultures and tissues using silica spin-column technology. In the presence of optimized chaotropic binding conditions, RNA binds to a silica membrane while contaminants are removed through sequential wash steps, producing RNA suitable for sensitive downstream workflows.  Each column supports a high binding capacity (up to 20 µg) for efficient recovery from a wide range of input amounts.  For applications that require minimal genomic DNA carryover, the kit can be used with an optional on-column DNase digestion using a dedicated DNase-on-column buffer, after which the DNase is removed during wash steps.  Purified RNA is eluted in DNase/RNase/Protease-free water (or low-salt TE buffer), ready for immediate use or storage.  🍁 Researched, Developed and Manufactured in London, ON, Canada.  Features High-purity total RNA isolation from mammalian cells and tissues Silica spin columns with up to 20 µg binding capacity  Optional on-column DNase digestion to minimize genomic DNA contamination  Efficient removal of proteins, salts, and inhibitors via multi-step washing  Fast, streamlined workflow without phenol/chloroform extraction  Cost-effective performance without compromising yield or purity Applications Total RNA extraction from mammalian cell cultures Total RNA extraction from mammalian tissues RT-qPCR / RT-PCR and gene expression analysis  RNA-seq / NGS library preparation (after QC and DNase option as needed) cDNA synthesis and downstream transcript profiling General RNA workflows requiring low inhibitor carryover  Included in the Kit 50 x DNase/RNase/Protease Free columns Lysis Buffer B Wash Buffer X Wash Buffer XB DNase/RNase/Protease-Free Water Protocol  Documents SDS Sheets* * Contact us for SDS sheets or COAs For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Mammalian Genomic DNA Purification Kit Delivers High Yield and Purity Designed to deliver exceptional purity and yield, this kit guarantees results that are on par with market leaders. Streamline your DNA extraction workflow and unlock the full potential of your samples without breaking the bank. With HoppeSyler's unparalleled cost savings to quality ratio, you can trust that your genomic DNA isolation needs are met with precision, reliability, and affordability. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features High-quality genomic DNA isolation and purification Comparable purity and yield to market leaders Efficient removal of contaminants and inhibitors Streamlined protocol for fast and reliable results Cost-effective solution without compromising quality Applications Genomic DNA extraction from cells or tissues PCR amplification of target genes Genotyping studies Genetic screening and profiling Next-generation sequencing (NGS) library preparation Included in the Kit 50 x DNase/RNase/Protease Free columns Lysis Buffer A Wash Buffer A Elution Buffer A Proteinase K Protocol  Documents SDS Sheets* Contact us for SDS sheets or COAs For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler High Fidelity DNA Polymerase is a recombinant polymerase that contains a 5'->3' DNA polymerase and a 3'->5' exonuclease domains along with a Sso7D tethering domain. The High-Fidelity DNA polymerase achieves 55-fold higher fidelity than Taq DNA polymerase and generates blunt end PCR products. The High-Fidelity DNA polymerase can generate DNA fragments up to 10 Kbp in length with higher accuracy and two-to-three-fold greater processivity than Taq DNA polymerase. Source High Fidelity DNA Polymerase is a recombinant polymerase derived from the archaeon Pyrococcus furiosus. 🍁 Researched, Developed and Manufactured in London, ON, Canada.    Catalogue Number Units Number of Reactions M0401-002 200 U 80 M0401-010 1000 U 400 M0401-050 5000 U 2000   Included in the Kit HoppeSyler High Fidelity DNA Polymerase GC Enhancer (For GC-rich or difficult to amplify templates) 5X High Fidelity PCR Buffer Protocol Features High fidelity amplification (> 55X Higher than Taq) Robust and reliable amplification Tolerates a wide range of templates 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Resistant to contaminated templates, such as plant DNA Amplification confirmed up to 10 kb in PCR reactions Superior performance for a broad range of templates (High AT to high GC rich) For Research Use Only. Not for use in diagnostic procedures.

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Nucleic Acid and Protein Decontamination G-Lock is a next-generation laboratory decontamination spray designed to inactivate and remove trace biomolecular contaminants (DNA, RNA, and proteins) from routinely used surfaces and equipment. 🍁 Developed and Manufactured in Canada. Use is straightforward: Spray the surface. Allow a brief dwell. Then rinse and wipe clean. G-Lock is designed for molecular biology environments where even tiny carryover can compromise PCR, qPCR, sequencing, or immunodetection workflows; it actively breaks down nucleic acids and proteins (including RNases) so they become non-amplifiable and easy to remove, helping maintain clean, amplification-ready workspaces. G-Lock has been evaluated against a broad range of biomolecules, including DNA and RNA, and is designed to reduce nucleic acid contamination to levels compatible with highly sensitive PCR and qPCR. It is also formulated to neutralize persistent enzyme residues and can inactivate residual RNase on benches, pipettes, and instrument surfaces, supporting RNase-controlled environments for RNA workflows. SafetyWARNING. Flammable liquid and vapour. Causes serious eye irritation.DocumentsSafety Data Sheet, G-Lock DNA & Protein Decontamination Spray (PDF)For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Tris Buffer, (pH 10.0 or pH 8.0 available), is manufactured to the highest molecular biology standards, ensuring consistency and reliability in sensitive applications. This 1 M solution, adjusted to pH 8.0 or 10.0, is sterile-filtered and certified free of DNase, RNase, and protease contamination. Designed for use in a wide range of molecular biology workflows, it provides a stable buffering environment that supports nucleic acid integrity and enzymatic activity. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Catalogue Number Number of Bottles  pH Volume MBTRIS-P8 1 8.0 500 mL  MBTRIS-P10 1 10.0 500 mL    Features 1 M Tris-HCl buffer Sterile, endotoxin-free, and enzyme-free (DNase/RNase/Protease free) Prepared with ultra-pure reagents and Type I water Lot-specific Certificate of Analysis (CoA) provided Manufactured in Canada under strict QC standards Applications PCR/qPCR and RT-PCR reactions Preparation of electrophoresis buffers (TAE, TBE) RNA and DNA resuspension or dilution Enzyme reactions requiring nuclease/protease-free conditions General molecular biology and biochemistry workflows   SafetypH 10.0: WARNING. Causes serious eye irritation. pH 8.0 is not classified.DocumentsSafety Data Sheet, 1M Tris-HCl, pH 10.0 (PDF) For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Dulbecco’s Phosphate Buffered Saline (D-PBS) is an isotonic, balanced salt solution formulated to support cell culture and molecular biology workflows. It contains sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate to maintain physiological osmolarity and pH. The buffer is sterile-filtered and free from calcium, magnesium, DNase, RNase, and protease contamination, ensuring reproducibility and protection of sensitive samples. 🍁 Researched, Developed and Manufactured in London, ON, Canada.   Catalogue Number Number of Bottles  Volume MBPBS-500 1 500 mL  MBPBS-P500 10 10 x 500 mL    Features 1X D-PBS, pH 7.4 (±0.1) Sterile, endotoxin-tested, and enzyme-free (DNase/RNase/Protease free) Without calcium or magnesium to prevent unwanted cell signalling or aggregation Manufactured with high-purity reagents and Type I water Lot-specific Certificate of Analysis (CoA) provided Made in Canada under strict QC standards Applications Washing and rinsing adherent or suspension cells Dilution of reagents, enzymes, or compounds in cell culture Preparation of samples for flow cytometry, microscopy, or nucleic acid work Maintaining osmotic balance in cell- and tissue-based assays For Research Use Only. Not for use in diagnostic procedures.

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