HoppeSyler 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The HoppeSyler Hot Start Taq Master Mix offers exceptional value per unit in terms of cost. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.   🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Number of Reactions Volume (mL) M0602-005 50 1.25 M0602-020 200 5.0   Included in the Kit HoppeSyler 2X Hot Start Taq Master Mix GC Enhancer (For GC-rich or difficult to amplify templates) Protocol Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle Hot-Start technology prevents the formation of non-specific products in PCR For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler M-Lock Mycoplasma Prevention Spray is a ready-to-use laboratory surface spray for maintaining clean laboratory work areas. It is designed for routine use on clean, solid laboratory surfaces and equipment (incubators, hoods, bench-tops, storage containers, and instruments) as part of a good-laboratory-practice cleaning routine. 🍁 Developed and Manufactured in Canada. Instructions for Use Spray directly onto the target surface, ensuring even coverage. Wipe the surface dry using a clean paper towel or disposable cloth. M-Lock is supplied ready to use and is formulated for everyday laboratory workflows. SafetyDANGER. Highly flammable liquid and vapour. Causes serious eye damage. May cause allergy or asthma symptoms or breathing difficulties if inhaled. May cause drowsiness or dizziness.DocumentsSafety Data Sheet, M-Lock Mycoplasma Prevention Spray (PDF)For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler dNTP Mix (10 mM each) is a ready-to-use aqueous solution of dATP, dTTP, dCTP and dGTP at a concentration of 10 mM. The individual bases are at a stock concentration of 100 mM, and then are mixed in RNase/DNase/Protease free water to achieve indicated concentrations. The purity of these bases is > 99 %, as verified by HPLC.   🍁 Researched, Developed and Manufactured in London, ON, Canada. Storage conditions  All components are to be stored at -20 °C. These components can be thawed at 4 °C prior to use.   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA, producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis. 🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Units Number of Reactions M0202-010 1000 U 400 M0202-050 5000 U 2000 M0202-500 50,000 U 20,000   Included in the Kit 10X PCR Buffer 50 mM Magnesium Sulfate GC Enhancer (For GC-rich or difficult to amplify templates) Protocol Features Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products Stable at room temperature for up to 2 weeks Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR Effective in DNA labeling For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler 2X Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The HoppeSyler Taq Master Mix offers exceptional value per unit in terms of cost.  🍁 Researched, Developed and Manufactured in London, ON, Canada.   Included in the Kit HoppeSyler 2X Taq Master Mix GC Enhancer (For GC-rich or difficult to amplify templates) Protocol  Catalogue Number Number of Reactions Volume (mL) M0501-005 50 1.25 M0501-020 200 5.0 Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler M4 Taq DNA Polymerase is an engineered, full-length thermostable DNA polymerase developed for fast, robust endpoint PCR across a wide range of sample types. It is supplied only in hot start format: the enzyme is inactive during reaction setup and becomes active under PCR cycling conditions, which improves specificity and reduces primer-dimer and non-specific amplification. M4 is optimized for fast cycling, high product yield and tolerance of common PCR inhibitors, and amplifies directly from bacterial colonies, overnight cultures, crude lysates and other challenging inputs. Because M4 extends faster than conventional Taq, protocols written for other polymerases should not be used unchanged: start from the Quick Guide settings in the datasheet.🍁 Researched, Developed and Manufactured in London, ON, Canada. Catalogue Number Units Number of Reactions M0902-010 1,000 U 400 M0902-050 5,000 U 2,000 Features Hot start built into the engineered enzyme: inactive during setup, fully active under cycling, no separate activation step Fast cycling: 15 s per kb extension; 2 kb amplified with a 20 s extension step Tolerates PCR inhibitors, including humic acid in soil extracts Direct PCR from bacterial colonies and overnight cultures Room-temperature stable up to 2 weeks Applications Colony PCR and direct PCR from culture Genotyping from crude lysates (for example mouse tail) Routine endpoint PCR Inhibitor-rich templates (soil-associated samples) Included in the Kit Component 1,000 U 5,000 U M4 Taq DNA Polymerase (5 U/µL) 1 × 200 µL 1 × 1 mL 5X M4 PCR Buffer (with MgCl₂) 4 × 1 mL 1 × 50 mL GC Enhancer 1 × 1 mL 1 × 10 mL Datasheet with protocol (PDF)GC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C.Lot-release testing: each polymerase lot is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity; lot-to-lot activity within ±5%.Documents Datasheet (PDF) Safety Data Sheet, M4 Taq DNA Polymerase (PDF) Safety Data Sheet, 5X M4 PCR Buffer (PDF) Safety Data Sheet, GC Enhancer (PDF) For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler 2X M4 Taq Master Mix with Dye is a ready-to-use 2X master mix formulated for fast PCR, high yield and strong specificity in standard endpoint workflows. It contains M4 Taq DNA Polymerase, dNTPs, MgCl₂, reaction buffer, stabilizers and a blue tracking dye in a single tube, reducing hands-on time and pipetting error. M4 is supplied only in hot start format: polymerase activity is suppressed during reaction setup and released under cycling (hot start is inherent to the engineered enzyme; no separate activation step), which reduces non-specific amplification and primer-dimer formation. Suited to rapid, reliable PCR from bacterial colonies, overnight cultures, crude-lysate genotyping samples, soil-associated samples and other routine templates; reactions load directly onto the gel. Because M4 extends faster than conventional Taq, protocols written for other polymerases should not be used unchanged: start from the Quick Guide settings in the datasheet.🍁 Researched, Developed and Manufactured in London, ON, Canada.Included in the Kit 2X M4 Taq Master Mix with Dye (4 × 1.25 mL) GC Enhancer (1 × 1 mL) Datasheet with protocol (PDF) GC Enhancer is provided for GC-rich and difficult templates. Store all components at −20 °C. Catalogue Number Number of Reactions Volume M0802-020 200 5.0 mL Features Hot start in a ready-to-use 2X format Fast cycling: 15 s per kb extension Tolerates common PCR inhibitors, including humic acid Direct PCR from bacterial colonies and cultures Blue tracking dye for direct gel loading Lot-release testing: the polymerase is tested for DNase activity, mycoplasma DNA, host genomic DNA and PCR activity before formulation; each master mix lot is tested before release.Documents Datasheet (PDF) Safety Data Sheet, 2X M4 Taq Master Mix with Dye (PDF) Safety Data Sheet, GC Enhancer (PDF) For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Ultra-Pure Sterile Water is specially prepared for molecular biology and life science applications requiring the highest purity. It is rigorously purified, autoclaved, and tested to ensure it is completely free of DNases, RNases, and proteases, making it ideal for sensitive workflows where nucleic acid or protein integrity must be preserved. 🍁 Researched, Developed and Manufactured in London, ON, Canada.   Catalogue Number Number of Bottles  Volume MBWTR-500 1 500 mL  MBWTR-P500 10 10 x 500 mL    Features Sterile, enzyme-free (DNase/RNase/Protease free) Endotoxin-tested and prepared with Type I water Certified Molecular Biology grade Lot-specific Certificate of Analysis (CoA) provided Produced in Canada under strict QC standards Applications Preparation of PCR/qPCR master mixes and buffers Dilution or resuspension of nucleic acids and proteins Preparation of enzyme reactions requiring nuclease-free conditions General molecular biology workflows requiring ultra-pure water   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler TE Buffer (Tris–EDTA) is formulated with 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA to provide a stable environment for nucleic acid storage and handling. The Tris component maintains a constant pH, while EDTA chelates divalent metal ions such as Mg²⁺ and Ca²⁺, protecting DNA and RNA from enzymatic degradation. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 (±0.1) Sterile, DNase/RNase/Protease free Manufactured with high-purity reagents and Type I water Lot-specific Certificate of Analysis (CoA) provided Made in Canada under strict QC standards Applications Long-term storage of DNA and RNA Resuspension of nucleic acid pellets after extraction Preparation of plasmid DNA for cloning, sequencing, or PCR Protection of nucleic acids from nuclease degradation   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler NaCl (5M) is a Sodium Chloride Solution, prepared using high-purity sodium chloride and ultra-pure water to ensure reliable performance in molecular biology workflows. The solution is sterile-filtered and free of DNase, RNase, and protease contamination, making it ideal for use in sensitive enzymatic and nucleic acid–based applications. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features 5 M NaCl solution (290.33 g/L) Sterile-filtered, enzyme-free (DNase/RNase/Protease free) Molecular Biology grade Lot-specific Certificate of Analysis (CoA) provided Manufactured in Canada under strict QC standards Applications Preparation of buffers and media Salt gradient preparation in protein and nucleic acid purification Hybridization buffers and nucleic acid precipitation General molecular biology and biochemistry applications    For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Taq DNA Polymerase reliably amplifies a wide variety of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA, producing consistent PCR products. This Taq polymerase leaves a 3' adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. 🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Units Number of Reactions M0101-010 1000 U 400 M0101-050 5000 U 2000 M0101-500 50,000 U 20,000   Features Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Stable at room temperature for up to 2 weeks Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR Effective in DNA labeling Included in the Kit 10X PCR Buffer 50 mM Magnesium Sulfate GC Enhancer (For GC-rich or difficult to amplify templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler PCR Cleanup Kit Delivers Efficient and Cost-Effective Cleanup This kit delivers exceptional purification results, meeting or exceeding the performance of renowned market leaders. Replace tedious manual purification methods with HoppeSyler’s PCR Cleanup Kit for a faster, more efficient workflow. Obtain high-purity PCR products while saving both time and resources. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features Efficient PCR product purification High-quality purification comparable to market leaders Rapid and reliable removal of contaminants and inhibitors User-friendly protocol for streamlined workflow Outstanding cost-effectiveness without compromising quality Applications Purification of PCR amplicons DNA sequencing cleanup Genotyping and SNP analysis Cloning and molecular cloning Fragment analysis Included in the Kit 50 x DNase/RNase/Protease Free columns Dilution Buffer A Wash Buffer A Elution Buffer A Protocol  Documents SDS Sheets* * Contact us for SDS sheets or COAs For Research Use Only. Not for use in diagnostic procedures.

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