CSR 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The CSR Hot Start Taq Master Mix offers exceptional value per unit in terms of cost. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.   🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Number of Reactions Volume (mL) M0602-005 50 1.25 M0602-020 200 5.0   Included in the Kit CSR 2X Hot Start Taq Master Mix GC Extender (For GC-rich or difficult to amplify templates) Protocol Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle Hot-Start technology prevents the formation of non-specific products in PCR Documents Product Information Sheet Quick Guide Sheet SDS Sheet For Research Use Only. Not for use in diagnostic procedures.

Read more less

Serum-Free, Ready-to-Use Cryopreservation Medium Trusted by Research Laboratories Worldwide Freezing mammalian cells is a routine but essential part of biological research. New cell lines are often banked quickly to protect against contamination, incubator failure, mix ups, or other surprises that can wipe out weeks (or months) of work. While many freezing methods exist, they all rely on a freezing medium containing a cryoprotectant. After cells are moved from growth medium into the freezing medium, they’re typically cooled at a controlled rate and stored either in liquid nitrogen vapor or in ultra low temperature storage.   In practice, the “standard” approach can become messy. Not every lab has ideal freezer capacity, and workflows that require serum, multiple washes, or tightly controlled freezing programs can introduce extra variability and extra hands on time.   Bambanker Cell Freezing Media were developed to streamline this process. The formulation is designed for straightforward cryopreservation at either −80°C or in liquid nitrogen, reducing reliance on complex controlled rate freezing steps and helping labs avoid adding another specialized freezer just to maintain a workflow. No programmed or stepwise freezing is needed. Bambanker reagents are serum free, supplied ready to use, and can be stored refrigerated for up to two years.   Strong Post-Thaw Recovery, Including Sensitive Cell Types When you thaw a vial, you want cells that are intact, healthy, and ready to work. Bambanker freezing reagents are built around a simple goal: make cryopreservation easy while maintaining high post-thaw viability. These reagents have been adopted by labs worldwide and were originally developed in Japan. They have also been evaluated across a large number of cell lines at JCRB, and published studies are available that document successful use in a range of applications.   In many cases, recovery from Bambanker preserved cells exceeds what labs see with conventional freezing media and typical protocols. Across cell types, survival is commonly above 50%, and many lines recover at ~90% or higher. Even challenging cells, including hematopoietic stem cells, can be cryopreserved effectively. Long term storage can also affect cell state, including unwanted differentiation. Bambanker reagents are formulated to help minimize these shifts.    Why Serum Free Matters for Consistency? Serum can be a major source of variability in cryopreservation. Media that include animal derived whole serum often show lot to lot differences in performance because serum composition (proteins, growth factors, and other biomolecules) can vary between batches. That variability can show up later as inconsistent recovery and inconsistent cell behavior, which can ripple into experimental reproducibility.   Bambanker products are manufactured without whole serum. Instead, ingredients are precisely specified, helping reduce batch driven variability and supporting more consistent thawing and recovery behaviour across vials and over time.   Key Features Serum-free formulation Ready-to-use cryopreservation medium Simple and reproducible freezing protocol Suitable for a wide range of mammalian cells Trusted by research laboratories worldwide   Documents Protocol SDS Sheet For Research Use Only. Not for use in diagnostic procedures.

Read more less

Like Coomassie Blue, only simplerAcquaStain is a one-step, Coomassie-style protein gel stain that eliminates the usual pre-washes and destaining. Just run your gel, add AcquaStain, and bands begin to appear within seconds. No post-treatment needed. AcquaStain is also a safer, easier alternative to many traditional dyes. It contains no harsh acids or hazardous components and is designed for straightforward disposal after use. Because it’s water-based, gels show no noticeable shrinkage or wrinkling, and proteins recovered from AcquaStain-stained gels remain fully compatible with mass spectrometry. One step, about 10 minutesMany “fast” staining systems rely on expensive equipment or sacrifice band clarity to save time. AcquaStain delivers speed and clean results without any external apparatus. Typical gels can be stained in about 10 minutes, and you can leave gels in the stain longer (even overnight) without worrying about over-staining. You get strong sensitivity with a crystal-clear gel background, so bands stand out sharply. AcquaStain is a teal, precipitate-free solution, and no shaking is required before use. Protocol for Mass spectrometry: Cut out protein band and place in microfuge tube Add 1 ml 30% ethanol or 30% acetone (30% acetic acid may be used but will result in acetylation of N-terminus). Incubate for 30 minutes. Repeat steps 2 and 3 until all stain removed from protein (typically 2 or 3 washes are required for complete stain removal) Process as typical for mass spec analysis. Ideal for teaching labsAcquaStain makes it easy to run and stain gels within a standard lab class period, making it a great fit for teaching environments where time, simplicity, and consistent results matter. Documents Protocol SDS Sheet For Research Use Only. Not for use in diagnostic procedures.

Read more less

Eliminate Bio-molecular Contamination G-Lock is a next-generation laboratory decontamination spray designed to inactivate and remove trace contaminants such as DNA, RNA, proteins, and common laboratory microbes from routinely used surfaces and equipment. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Use is straightforward: Spray the surface Allow brief contact time Then rinse and wipe clean G-Lock is designed for molecular biology environments where even tiny carryover can compromise PCR, qPCR, sequencing, or immunodetection workflows; it actively breaks down nucleic acids and proteins so they become non-amplifiable and easy to remove, helping maintain clean, amplification-ready workspaces. It is intended for routine lab decontamination against common contaminants, including typical research-associated bacteria such as E. coli, but it is not positioned for highly resistant organisms such as mycobacteria (for that application, use a dedicated mycobacteria-targeted product). G-Lock has been evaluated against a broad range of biomolecules, including DNA and RNA, and is designed to reduce nucleic acid contamination to levels compatible with highly sensitive PCR and qPCR. It is also formulated to neutralize persistent enzyme residues and can inactivate residual RNase on benches, pipettes, and instrument surfaces, supporting RNase-controlled environments for RNA workflows. In addition to biomolecules, G-Lock helps eliminate common laboratory microbes under laboratory test conditions, improving both contamination control and overall lab hygiene. Documents SDS Sheet For Research Use Only. Not for use in diagnostic procedures.

Read more less

CSR High Fidelity DNA Polymerase is a recombinant polymerase that contains a 5'->3' DNA polymerase and a 3'->5' exonuclease domains along with a Sso7D tethering domain. The High-Fidelity DNA polymerase achieves 55-fold higher fidelity than Taq DNA polymerase and generates blunt end PCR products. The High-Fidelity DNA polymerase can generate DNA fragments up to 10 Kbp in length with higher accuracy and two-to-three-fold greater processivity than Taq DNA polymerase. Source High Fidelity DNA Polymerase is a recombinant polymerase derived from the archaeon Pyrococcus furiosus. 🍁 Researched, Developed and Manufactured in London, ON, Canada.    Catalogue Number Units Number of Reactions M0401-002 200 U 80 M0401-010 1000 U 400 M0401-050 5000 U 2000   Included in the Kit CSR High Fidelity DNA Polymerase GC Extender (For GC-rich or difficult to amplify templates) 5X High Fidelity PCR Buffer Protocol Features High fidelity amplification (> 55X Higher than Taq) Robust and reliable amplification Tolerates a wide range of templates 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Resistant to contaminated templates, such as plant DNA Amplification confirmed up to 10 kb in PCR reactions Superior performance for a broad range of templates (High AT to high GC rich) Documents Product Information Sheet Quick Guide Sheet SDS Sheet For Research Use Only. Not for use in diagnostic procedures.

Read more less

CleanNGS magnetic bead cleanup kit Fast, consistent DNA/RNA purification for sequencing workflows CleanNGS is a magnetic bead-based cleanup system designed for NGS and Sanger sample and library prep. It supports both routine cleanup and size selection, helping you remove salts, primers, and small byproducts while keeping the fragments you actually want. Why labs use it High recovery, low carryover: clean eluates with minimal contaminants.Quick magnetic response: beads separate efficiently for shorter hands-on time.Stable, easy workflow: a simple, repeatable protocol that works manually or on a liquid handler.Cost-conscious alternative: built to drop into SPRI-style cleanup workflows without expensive consumables. Reliable size selection, without the guessing game Size selection with bead chemistry depends on the binding environment. CleanNGS includes pre-formulated solutions designed to deliver tight, reproducible selection for: Left-side selection (remove small fragments) Right-side selection (remove large fragments) Double-sided selection (narrow fragment ranges) That means fewer re-optimizations and more consistent libraries run-to-run. One kit for DNA and RNA CleanNGS is produced RNase-free, so the same kit can be used for DNA workflows and RNA applications where cleanliness matters. Automation-ready CleanNGS is well suited for high-throughput cleanup on common liquid handling platforms. It can be integrated into automated protocols to streamline repetitive NGS purification steps, while maintaining flexibility for day-to-day variation in sample types and volumes. Documents User Manual SDS Brochure Application Notes: NGS Library Preparation CleanNGS bead cleanup performs equal compared to the lllumina SPB or Ampure XP competitor kits CleanNGS: Purification for Next Generation Sequencing Library Preparations For Research Use Only. Not for use in diagnostic procedures.   Customer Feedback: “It looks to me like your CleanNGS beads work just as well as [competitor beads]. They are convenient as they are already prepared and can be ordered in smaller quantities.”Name and institution withheld upon request, 2023 “I plan to replace [competitor beads] with CleanNGS Beads for cleaning PCR products and NGS library.”Hung, UNL, 2023 “We have tested the [CleanNGS] beads and it worked great for us. I am going to place an order.”Yinan, MYU, 2022

Read more less