HoppeSyler 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The HoppeSyler Hot Start Taq Master Mix offers exceptional value per unit in terms of cost. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.   🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Number of Reactions Volume (mL) M0602-005 50 1.25 M0602-020 200 5.0   Included in the Kit HoppeSyler 2X Hot Start Taq Master Mix GC Extender (For GC-rich or difficult to amplify templates) Protocol Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle Hot-Start technology prevents the formation of non-specific products in PCR For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler dNTP Mix (10 mM each) is a ready-to-use aqueous solution of dATP, dTTP, dCTP and dGTP at a concentration of 10 mM. The individual bases are at a stock concentration of 100 mM, and then are mixed in RNase/DNase/Protease free water to achieve indicated concentrations. The purity of these bases is > 99 %, as verified by HPLC.   🍁 Researched, Developed and Manufactured in London, ON, Canada. Storage conditions  All components are to be stored at -20 °C. These components can be thawed at 4 °C prior to use.   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler M-Lock is a Mycoplasma prevention spray that provides a fast, reliable, and convenient solution for maintaining contamination-free cell culture environments. Unlike traditional cleaning methods—such as wiping with sponges or paper towels, which risk spreading microbes from one surface to another— M-Lock acts directly at the source. Its powerful formulation targets and eliminates a broad spectrum of laboratory contaminants, including mycoplasma, bacteria, and viruses, helping to safeguard your valuable experiments. 🍁 Developed and Manufactured in Canada. Features Eradicates mycoplasma Non-corrosive and non-carcinogenic Convenient and stable Ready-to-use Instructions for Use Spray the Mycoplasma prevention solution directly onto the desired surface, ensuring even coverage Allow the solution to sit for 10 minutes to maximize its effectiveness Wipe the surface dry using a clean paper towel or disposable cloth What are mycoplasma? Mycoplasma are especially troublesome because they lack a cell wall and evade many antibiotic treatments. Once inside cell culture samples, they can silently compromise experimental data. The best strategy is prevention—and that’s where M-Lock excels. Designed for routine use in incubators, hoods, bench-tops, storage containers, and instruments, M-Lock offers comprehensive protection against unwanted microbial invaders. Using M-Lock is simple: spray directly onto surfaces and equipment, allow 10 minutes for action, and the treated area is effectively decontaminated. This ready-to-use solution is non-corrosive, non-carcinogenic, and formulated for everyday laboratory workflows. Regular use of M-Lock significantly reduces the risk of contamination events, protecting both your cells and your research. With M-Lock, labs can achieve peace of mind knowing their culture areas remain free of hidden threats—quickly, easily, and cost-effectively. For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. Hot Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis. 🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Units Number of Reactions M0202-010 1000 U 400 M0202-050 5000 U 2000 M0202-500 50,000 U 20,000   Included in the Kit 10X PCR Buffer 50 mM Magnesium Sulfate GC Extender (For GC-rich templates and difficult templates) Protocol Features Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products Stable at room temperature for up to 2 weeks Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR Effective in DNA labeling For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler 2X Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The HoppeSyler Taq Master Mix offers exceptional value per unit in terms of cost.  🍁 Researched, Developed and Manufactured in London, ON, Canada.   Included in the Kit HoppeSyler 2X Taq Master Mix GC Extender (For GC-rich or difficult to amplify templates) Protocol  Catalogue Number Number of Reactions Volume (mL) M0501-005 50 1.25 M0501-020 200 5.0 Features Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Ultra-Pure Sterile Water is specially prepared for molecular biology and life science applications requiring the highest purity. It is rigorously purified, autoclaved, and tested to ensure it is completely free of DNases, RNases, and proteases, making it ideal for sensitive workflows where nucleic acid or protein integrity must be preserved. 🍁 Researched, Developed and Manufactured in London, ON, Canada.   Catalogue Number Number of Bottles  Volume MBWTR-500 1 500 mL  MBWTR-P500 10 10 x 500 mL    Features Sterile, enzyme-free (DNase/RNase/Protease free) Endotoxin-tested and prepared with Type I water Certified Molecular Biology grade Lot-specific Certificate of Analysis (CoA) provided Produced in Canada under strict QC standards Applications Preparation of PCR/qPCR master mixes and buffers Dilution or resuspension of nucleic acids and proteins Preparation of enzyme reactions requiring nuclease-free conditions General molecular biology workflows requiring ultra-pure water   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler TE Buffer (Tris–EDTA) is formulated with 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA to provide a stable environment for nucleic acid storage and handling. The Tris component maintains a constant pH, while EDTA chelates divalent metal ions such as Mg²⁺ and Ca²⁺, protecting DNA and RNA from enzymatic degradation. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 (±0.1) Sterile, DNase/RNase/Protease free Manufactured with high-purity reagents and Type I water Lot-specific Certificate of Analysis (CoA) provided Made in Canada under strict QC standards Applications Long-term storage of DNA and RNA Resuspension of nucleic acid pellets after extraction Preparation of plasmid DNA for cloning, sequencing, or PCR Protection of nucleic acids from nuclease degradation   For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler NaCl (5M) is a Sodium Chloride Solution, prepared using high-purity sodium chloride and ultra-pure water to ensure reliable performance in molecular biology workflows. The solution is sterile-filtered and free of DNase, RNase, and protease contamination, making it ideal for use in sensitive enzymatic and nucleic acid–based applications. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features 5 M NaCl solution (290.33 g/L) Sterile-filtered, enzyme-free (DNase/RNase/Protease free) Molecular Biology grade Lot-specific Certificate of Analysis (CoA) provided Manufactured in Canada under strict QC standards Applications Preparation of buffers and media Salt gradient preparation in protein and nucleic acid purification Hybridization buffers and nucleic acid precipitation General molecular biology and biochemistry applications    For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This Taq polymerase leaves a 3' adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. 🍁 Researched, Developed and Manufactured in London, ON, Canada.  Catalogue Number Units Number of Reactions M0101-010 1000 U 400 M0101-050 5000 U 2000 M0101-500 50,000 U 20,000   Features Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Stable at room temperature for up to 2 weeks Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR Effective in DNA labeling Included in the Kit 10X PCR Buffer 50 mM Magnesium Sulfate GC Extender (For GC-rich templates and difficult templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler ROBUST DNA Polymerase is a highly efficient next-generation DNA polymerase offering enhanced processivity and yield compared to Taq DNA polymerase. This enzyme enables shorter extension times and fewer cycles to achieve equivalent levels of amplification. It also features significantly improved resistance to various PCR inhibitors, chemical denaturants, and increased thermal stability, maintaining activity even after 1 hour at 95 °C. Its enhanced robustness allows for the use of lower-quality template DNA and simplifies DNA purification protocols. The ROBUST DNA Polymerase utilizes aptamer-based hot start technology, providing reversible inhibition that prevents primer-dimer formation and non-specific amplification, enabling reaction setup at room temperature. Hot-Start Technology The HoppeSyler ROBUST DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3' cap that prevents its amplification and ensures no interference with cloning or any downstream analysis. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Included in the Kit HoppeSyler ROBUST DNA Polymerase 10X ROBUST PCR Buffer 50 mM Magnesium Sulfate GC Extender (For GC-rich or difficult to amplify templates) Protocol  Catalogue Number Units Number of Reactions M0301-002 200 U 80 M0301-010 1000 U 400 M0301-050 5000 U 2000   Features Robust and reliable reactions Compatible with contaminated templates, such as plant DNA Compatible with direct colony PCR 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle Hot Start technology prevents the formation of non-specific products in PCR Stable at room temperature for up to 2 weeks For Research Use Only. Not for use in diagnostic procedures.

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HoppeSyler PCR Cleanup Kit Delivers Efficient and Cost-Effective Cleanup This kit delivers exceptional purification results, meeting or exceeding the performance of renowned market leaders. Replace tedious manual purification methods with HoppeSyler’s PCR Cleanup Kit for a faster, more efficient workflow. Obtain high-purity PCR products while saving both time and resources. 🍁 Researched, Developed and Manufactured in London, ON, Canada. Features Efficient PCR product purification High-quality purification comparable to market leaders Rapid and reliable removal of contaminants and inhibitors User-friendly protocol for streamlined workflow Outstanding cost-effectiveness without compromising quality Applications Purification of PCR amplicons DNA sequencing cleanup Genotyping and SNP analysis Cloning and molecular cloning Fragment analysis Included in the Kit 50 x DNase/RNase/Protease Free columns Dilution Buffer A Wash Buffer A Elution Buffer A Protocol  Documents SDS Sheets* * Contact us for SDS sheets or COAs For Research Use Only. Not for use in diagnostic procedures.

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The HoppeSyler Mammalian RNA Purification Kit provides a rapid, robust method to purify high-quality total RNA from mammalian cell cultures and tissues using silica spin-column technology. In the presence of optimized chaotropic binding conditions, RNA binds to a silica membrane while contaminants are removed through sequential wash steps, producing RNA suitable for sensitive downstream workflows.  Each column supports a high binding capacity (up to 20 µg) for efficient recovery from a wide range of input amounts.  For applications that require minimal genomic DNA carryover, the kit can be used with an optional on-column DNase digestion using a dedicated DNase-on-column buffer, after which the DNase is removed during wash steps.  Purified RNA is eluted in DNase/RNase/Protease-free water (or low-salt TE buffer), ready for immediate use or storage.  🍁 Researched, Developed and Manufactured in London, ON, Canada.  Features High-purity total RNA isolation from mammalian cells and tissues Silica spin columns with up to 20 µg binding capacity  Optional on-column DNase digestion to minimize genomic DNA contamination  Efficient removal of proteins, salts, and inhibitors via multi-step washing  Fast, streamlined workflow without phenol/chloroform extraction  Cost-effective performance without compromising yield or purity Applications Total RNA extraction from mammalian cell cultures Total RNA extraction from mammalian tissues RT-qPCR / RT-PCR and gene expression analysis  RNA-seq / NGS library preparation (after QC and DNase option as needed) cDNA synthesis and downstream transcript profiling General RNA workflows requiring low inhibitor carryover  Included in the Kit 50 x DNase/RNase/Protease Free columns Lysis Buffer B Wash Buffer X Wash Buffer XB DNase/RNase/Protease-Free Water Protocol  Documents SDS Sheets* * Contact us for SDS sheets or COAs For Research Use Only. Not for use in diagnostic procedures.

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