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🍁 CSR 2X Hot Start Taq Master Mix (with Dye)

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    CSR 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of... Read more

    $29.00

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      Description

      CSR 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 3′ adenine overhang, making it ideal for TA cloning. The CSR Hot Start Taq Master Mix offers exceptional value per unit in terms of cost.

      Hot Start Technology
      Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.

       

      🍁 Researched, Developed and Manufactured in London, ON, Canada.

       Catalogue Number Number of Reactions Volume (mL)
      M0602-005 50 1.25
      M0602-020 200 5.0

       

      Included in the Kit
      • CSR 2X Hot Start Taq Master Mix
      • GC Extender (For GC-rich or difficult to amplify templates)
      • Protocol

      Features
      • Robust and reliable reactions
      • Tolerates a wide range of templates
      • Amplification confirmed up to 5 kb in PCR reactions
      • The error rate in PCR is 2.2 x 10 -5 errors per nucleotide per cycle
      • Hot-Start technology prevents the formation of non-specific products in PCR
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      • Species of Origin: Thermus aquaticus
      • Enzyme Type: Thermostable DNA polymerase
      • Format: 2X Master Mix, includes dNTPs
      • Magnesium Concentration: 3 mM
      • 5’->3′ Exonuclease Activity: Yes
      • “A” Overhang: Yes
      • 3’->′ Exonuclease Activity: No
      • Processivity Rate: 1 Kbp / minute
      • Amplification Limit: confirmed to 5 Kbp
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