CSR ROBUST DNA Polymerase is a highly efficient next-generation DNA polymerase offering enhanced processivity and yield compared to Taq DNA polymerase. This enzyme enables shorter extension times and fewer cycles to achieve equivalent levels of amplification. It also features significantly improved resistance to various PCR inhibitors, chemical denaturants, and increased thermal stability, maintaining activity even after 1 hour at 95 °C. Its enhanced robustness allows for the use of lower-quality template DNA and simplifies DNA purification protocols. The ROBUST DNA Polymerase utilizes aptamer-based hot start technology, providing reversible inhibition that prevents primer-dimer formation and non-specific amplification, enabling reaction setup at room temperature.
Hot-Start Technology
The CSR ROBUST DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.
🍁 Researched, Developed and Manufactured in London, ON, Canada.
Included in the Kit
- CSR ROBUST DNA Polymerase
- 50 mM Magnesium Sulfate
- GC Extender (For GC-rich or difficult to amplify templates)
- Protocol
| Catalogue Number |
Units |
Number of Reactions |
| M0301-002 |
200 U |
80 |
| M0301-010 |
1000 U |
400 |
| M0301-050 |
5000 U |
2000 |
Specification
- Robust and reliable reactions
- Compatible with contaminated templates, such as plant DNA.
- Compatible with direct colony PCR.
- 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb)
- Tolerates a wide range of templates
- Amplification confirmed up to 5 kb in PCR reactions
- The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle
- Hot Start technology prevents the formation of non-specific products in PCR
- Stable at room temperature for up to 2 weeks.
For Research Use Only. Not for use in diagnostic procedures.
