CSR Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templatesâincluding mouse, human, plasmid, and bisulfite-converted DNAâproducing consistent PCR products. This polymerase leaves a 3Ⲡadenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.
Hot Start Technology
Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3â cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.
đ Researched, Developed and Manufactured in London, ON, Canada.
| Â Catalogue Number |
Units |
Number of Reactions |
| M0202-010 |
1000 U |
400 |
| M0202-050 |
5000 U |
2000 |
| M0202-500 |
50,000 U |
20,000 |
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Kit Components
- 10X PCR Buffer
- 50 mM Magnesium Sulfate
- GC Extender (For GC-rich templates and difficult templates)
- Protocol
Specifications
- Generates PCR product with 3'-dA overhangs
- The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle
- Generates PCR product of up to 5 kb
- Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products
- Stable at room temperature for up to 2 weeks.Â
Applications
- Effective in routine and high throughput PCR
- Incorporates modified nucleotides in PCR.
- Effective in DNA labeling.
