Description

The CSR RNA Extraction Kit provides a rapid, robust method to purify high-quality total RNA from mammalian cell cultures and tissues using silica spin-column technology. In the presence of optimized chaotropic binding conditions, RNA binds to a silica membrane while contaminants are removed through sequential wash steps, producing RNA suitable for sensitive downstream workflows. 

Each column supports a high binding capacity (up to 20 µg) for efficient recovery from a wide range of input amounts.  For applications that require minimal genomic DNA carryover, the kit can be used with an optional on-column DNase digestion using a dedicated DNase-on-column buffer, after which the DNase is removed during wash steps.  Purified RNA is eluted in DNase/RNase/Protease-free water (or low-salt TE buffer), ready for immediate use or storage. 

🍁 Researched, Developed and Manufactured in London, ON, Canada.

 Features

• High-purity total RNA isolation from mammalian cells and tissues
• Silica spin columns with up to 20 µg binding capacity 
• Optional on-column DNase digestion to minimize genomic DNA contamination 
• Efficient removal of proteins, salts, and inhibitors via multi-step washing 
• Fast, streamlined workflow without phenol/chloroform extraction 
• Cost-effective performance without compromising yield or purity

Applications

• Total RNA extraction from mammalian cell cultures
• Total RNA extraction from mammalian tissues
• RT-qPCR / RT-PCR and gene expression analysis 
• RNA-seq / NGS library preparation (after QC and DNase option as needed)
• cDNA synthesis and downstream transcript profiling
• General RNA workflows requiring low inhibitor carryover 

Documents

• Protocol
• SDS Sheets*

* Contact us for SDS sheets or COAs

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Number of Preps: 50 preps
• Application: For the extraction of RNA from mammalian cells & tissues. Not for human diagnostic use. For research use only.
• Storage: 1 year at room temperature