Description

CSR Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This Taq polymerase leaves a 3' adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.

🍁 Researched, Developed and Manufactured in London, ON, Canada.

Features

• Generates PCR product with 3'-dA overhangs
• The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle
• Generates PCR product of up to 5 kb
• Stable at room temperature for up to 2 weeks

• Effective in routine and high throughput PCR
• Incorporates modified nucleotides in PCR
• Effective in DNA labeling

Specifications

• Species of Origin: Thermus aquaticus
• Enzyme Type: Thermostable DNA polymerase
• 5'->3' Exonuclease Activity: Yes
• “A” Overhang: Yes
• 3'->5' Exonuclease Activity: No
• Processivity Rate: 1 Kbp / minute
• Unit Definition: One unit incorporates 10 nmol of dNTP into acid-precipitable material in 30 minutes at 74°C.
• Amplification Limit: confirmed to 5 Kbp
• Enzyme Concentration: 5 units/µL
• Reaction Buffer (included): Yes. 10x concentration (no dNTPs). Magnesium sulfate is provided separately.
• GC Enhancer: Yes
• DNase Free: Yes
• RNase Free: Yes
• Protease Free: Yes
• Mycoplasma gDNA Free: Yes, measured at <10 copies per unit of enzyme
• E. coli gDNA Reduced: Yes, Measured at >10 copies per unit of enzyme
• SYBR™ Green Compatible: Yes
• Storage: 1 year at -20 °C
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